Tissue engineering is a multidisciplinary area that concerns with the principles of engineering and biological science to develop appropriate alternatives that improve, maintain and/or restore tissue’s biological function (O’Brien 2011), as shown in Fig. 2. Recently, the biodegradable polymers are playing a crucial role in tissue engineering field, because it facilitates removal of the implant without surgery. Thus, much attention has been given to the development of biomaterials from biopolymers. In medical applications, biopolymers have been applied successfully for the treatment of ligament injury, blood vessels damage, skin graft, drug delivery and wound treatment, etc. (Khan et al. 2015). It is believed that intrinsic properties of biopolymers can be improved by appropriate process engineering methods for optimum results such as cell attachment, elongation, and proliferation. Biocompatibility is the ability of a material to perform with an appropriate host response in a specific application (Williams 2011). The rationale of conducting biocompatibility study is to determine the suitability of a biomaterial particularly for human use and to confirm its noncytotoxic effects as well (Ciapetti et al. 1993). It is a key factor for designing any scaffold for tissue engineering application because scaffold continuously interacts with the subject’s body during treatment. If this parameter is not taken into consideration, then the material used can infect application site.