Paul et al. (2014c) removed blood, egg yolk and chocolate stains from cloths by crude keratinase of Paenibacillus woosongensis TKB2 with the release of eco-friendly effluent. Manivasagan et al. (2014) reported the first reference to a marine actinobacterium Actinoallteichus sp. MA-32 as a producer of thermo- and detergent stable keratinase in a feather-based medium. The resultant activity of the enzyme and its stability to SDS, ethoxylated octylphenol, DMSO, sodium sulphite, and mercaptoethanol are indicative of its potential application in laundry detergent formulation. In our findings, we achieved remarkable removal of bloodstain from stained clothwithin 2 h of incubation with the crude keratinase produced by B. safensis LAU 13(Lateef et al. 2015a). In a study conducted by Zhang et al. (2016c), the production of surfactants and commercial detergents stable keratinase by a strain of Gibberella intermedia was reported. The enzyme completely removed bloodstain from stained cloth when combined with commercial detergents. Most recently, Reddy et al. (2017) reported the potential application of keratinase produced by B. pumilus GRK as biodetergent additive due to its high compatibility with commercial detergents and efficient bloodstain removal from stained cloth by the keratinase-mediated detergent without damaging the cloth quality. Keratinases are therefore essential ingredients that could be utilized as additives in detergent formulations for efficient removal of keratinous stains in aneco-friendly manner